New Valley University, Faculty of Veterinary MedicineNew Valley Veterinary Journal2786-02721120210101Comparison between conventional and recent culture methods for detection of fish mycobacteria1817722910.21608/nvvj.2021.177229ENMahmoud E.HatemDepartment of microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.Essam A.NasrDepartment of microbiology, Serum and Vaccine Research Institute, Cairo, Egypt.MohamedMarzoukDepartment of microbiology, Faculty of Veterinary Med., Cairo University, Giza, Egypt.Fatma A.MohamedDepartment of microbiology, National Institute of Oceanography and Fisheries, Hurgada, Egypt.Mahmoud H.MohamedDepartment of fish diseases, Faculty of Veterinary Medicine, New Valley University, New Valley 72511, Egypt.Journal Article20210612The present study aimed to isolate and identify mycobacteria from Nile tilapia and fresh water as well as ornamental fish and aquarium water in Sohag Assuit and Qena Governorates. Conventional and recent methods (MB-Redox system) were used for isolation and detection of mycobacteria with comparison between them regarding to the rate of isolation time for growth and growth character of colonies. Three isolates were isolated from fresh water and identified as unclassified atypical mycobacteria and four isolates were recovered from aquarium water and identified as M. fortuitum and M. marinum. Eleven isolates were isolated from goldfish and identified as M. marinum, M. fortuitum and unclassified atypical mycobacteria. The recovery rate of MB-Redox system for mycobacteria was 12%, while in case of L-J medium was 10.66%. The mean time for detection of M. marinum was 3-7 days and 7-10 days for MBRedox system and Lowenstein – Jensen medium, respectively. The mean time for detection of M. fortuitum was 1-3 days for MB-Redox system, while in case of L-J medium was 3-6 days. The mean time for detection of other unidentified atypical mycobacteria was 3-8 days for MB-Redox system, while in case of L-J medium was 3-14 days. The detection of mycobacterial growth on MB-Redox system at different times revealed that the color of MB- Redox tubes appear yellow at time of inoculation changed to pink color after 3 days. The colonies appear as red particles on the surface. After 15 days, the color of MB-Redox medium changed to yellow with red particles on the surface.https://nvvj.journals.ekb.eg/article_177229_c7e1fc2c5f95f164d206690a768c4dd3.pdfNew Valley University, Faculty of Veterinary MedicineNew Valley Veterinary Journal2786-02721120210101A novel bacterial infection in cultured Nile tilapia, Oreochromis niloticus in New Valley, Egypt91517752810.21608/nvvj.2021.177528ENMahmoud HashiemMohamedDepartment of Animal Medicine, Faculty of Veterinary Medicine, New Valley University, Egypt.Omnia HamedSalehDepartment of Animal Medicine, Faculty of Veterinary Medicine, New Valley University, Egypt.Mohamed Abd El-Aziz AhmedAbd El-GalilDepartment of Fish Diseases and Management, Faculty of Veterinary Medicine, Sohag University, Egypt.Journal Article20210510Pseudomonas and micrococcus <em>(Pseudomonas fluorescens and</em> <em>M. luteus) are</em> an emerging opportunistic fish pathogens that results in considerable economic losses among the infected fish. In the present study, a total number of 150 cultured Nile tilapia, <em>Oreochromis niloticus</em> were collected from concrete ponds farms in Alkharga oases, New Valley governorate during the period from March to July 2019 and subject to clinical and bacteriological examination. including excessive black colouration of skin, scales loss, skin ulcer and focal hemorrhages, exophthalmia, fin congestion and rot. Internally, the diseased fish had congested internal organs including liver, spleen, and kidney., The prevalence of <em>Pseudomonas fluorescens</em> and<em> M. luteus infection</em> was 6.7 % withe a total number of 10 isolates and 11.3% withe a total number of 17 isolates respectively. Were recovered from infected fish. The suspected <em>Pseudomonas fluorescens</em> isolates were biochemically similar while The suspected <em>M. luteus</em> isolates were biochemically similar, except for Arginine hydrolysis, Vogus-Proskauer and Urease tests. The isolation and biochemical characterizations suggested that the isolates belong to the genus <em>Pseudomonas</em>. And genus <em>micrococcus</em>. The recovered <em>M. luteus</em> isolates were pathogenic to <em>O. niloticus</em>, this is the first report of <em>M. luteus</em> infection in <em>O. niloticus</em> in Egypt.https://nvvj.journals.ekb.eg/article_177528_b7307ec89fa24226f081a19022c13430.pdfNew Valley University, Faculty of Veterinary MedicineNew Valley Veterinary Journal2786-02721120210101Microbiological Quality of Kareish cheeses162217753010.21608/nvvj.2021.177530ENTawfik Abdel RahmanElbassionyDepartment of Food Hygiene (Milk Hygiene), Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt.Khaled MohamedEl-KhawasDepartment of Food Hygiene (Milk Hygiene), Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt.Nadia MohamedMostafaAnimal Health Research Institute, Dokki, Giza, Cairo 12618, EgyptRania MohmedEwidaDepartment of food hygiene, Faculty of Veterinary Medicine, New Valley University, Egypt.Journal Article20210507This study aimed to evaluate kareish cheese sold in two cities in Egypt (New Valley and Cairo). A total of 105 kareish cheese samples were collected from New Valley, Cairo and different cheese factories (35 samples for each). The samples were examined for the occurrence of some food poisoning microorganisms as well as their quality. All samples were submitted for physical, chemical, and microbiological examination. The physical examination revealed that 100% of the examined samples were normal in color, odor, and consistency. The mean values of salinity and pH were 2.0±0.26 and 4.4±0.04, respectively. The positive % of coliforms, E. coli, and molds & yeasts were 42.8, 30.5, and 89.5%, respectively with the mean count 3.45±0.22, 1.92±0.22, and 4.62±0.13 cfu/g, respectively. Moreover, Staphylococcus aureus, Salmonella spp. and Listeria monocytogenes could not be detected in the examined kareish cheese samples. The results clarified that the high risk for humans that may be occurred by consumption of contaminated kareish cheese. Hygienic measures must be taken during the manufacture of this type of cheese.https://nvvj.journals.ekb.eg/article_177530_6a14d277e7ce27714706a49cd7e806f2.pdfNew Valley University, Faculty of Veterinary MedicineNew Valley Veterinary Journal2786-02721120210101Evaluation of the efficiency of the most common commercial disinfectants against some pathogens isolated from New-valley poultry farms "in-vitro studies."232817753110.21608/nvvj.2021.177531ENSotohy AhmedSotohyDepartment of Animal and environmental Hygiene, Faculty of Veterinary medicine, Assiut University.Asmaa AMobarezDepartment of Animal Hygiene and zoonoses, Faculty of Veterinary medicine, New Valley University.Mohamed SaidDiabDepartment of Animal Hygiene and zoonoses, Faculty of Veterinary medicine, New Valley University.Journal Article20210614This study was conducted to compare the efficiency of three commercially available disinfectants (Virkon S, T.H5 and Np50) against 3 bacterial isolates from New-valley broiler farms (Salmonella typhimurium, E. coli and Staph. aureus) in vitro. The evaluation was conducted at three consequent contact times 5, 15, 30 min. All tested disinfectants were diluted with sterile distilled water and applied at three different concentrations. The concentrations used were the manufacturer's instruction beside a higher and lower one. At the recommended concentration by the manufacturer, the most powerful disinfectant against all tested organisms was TH5 followed by Virkon S. On the other hand, NP50 was the weakest disinfectant. At 5 minutes, the reduction log showed by TH5 on S. typhimurium, E. coli, and S. aureus were, 3.6, 2.55, 3.54, respectively. Moreover, the reduction log for Virkon-S were 3.02, 2.21, and 3.37 on S. typhimurium, E. coli, and S. aureus, respectively. On the other hand, the reduction log induced by NP50 were,1.76, 1.91, 3.37 on S. typhimurium, E. coli, and S. aureus, respectively. At 15 minutes, the reduction log showed by TH5 on S. typhimurium, E. coli, and S. aureus were, 4.97, 3.59, 4.11, respectively. Moreover, the reduction log for Virkon-S were 4.97, 3.31, and 5.19 on S. typhimurium, E. coli, and S. aureus, respectively. On the other hand, the reduction log induced by NP50 were,2.82, 1.82, 4.27 on S. typhimurium, E. coli, and S. aureus, respectively. After 30 minutes, no growth was observed for S. aureus with 100% inhibition for TH5 while the reduction log for S. typhimurium, E. coli were, 7.32 and 7.16, respectively. Concerning Virkon-S the reduction log was, 6.5, 4.67 for S. typhimurium, E. coli, respectively while, S. aureus were completely inhibited with 100% inhibition. On the other hand, the reduction log induced by NP50 after 30 minutes were, 5.93, 3.4, 7.35 for S. typhimurium, E. coli, and S. aureus, respectively.https://nvvj.journals.ekb.eg/article_177531_36d99dd805bb744196fa3578b9fbb95b.pdfNew Valley University, Faculty of Veterinary MedicineNew Valley Veterinary Journal2786-02721120210101Biometrical Studies on the Ovaries of Slaughtered Egyptian Balady Does During different phases of Estrous Cycle and Seasons293517753310.21608/nvvj.2021.177533ENAhmed MamdouhOsmanDepartment of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.Hassan AbdelsabourHusseinDepartment of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.Mervat S.HassanDepartment of Theriogenology, Faculty of Veterinary Medicine, New Valley University.Journal Article20210513To study the biometrical data of the ovaries from slaughtered Balady does in relation to estrous cycle and seasons, a total number of 127 normal pair ovaries were taken for this purpose in Assiut province. The obtained materials were classified according to phases of estrus cycle (Proestrous 39, Estrus 12, Metestrus 23 and Diestrus 53) and as well according to seasons (Autumn 36, Winter 40, Spring 26 and Summer 25). The biometry of the ovaries included the length, breadth, thickness and weight. The surface follicles and corpora lutea were counted in each ovary and their diameters measured. The follicles were classified according to their size into: small (< 3 mm), medium (3-5 mm) and large (> 5 mm). The ovaries of does were small, almond shape and shiny. The right ovaries are significantly larger than the left (P<0.01). Among phases of estrous cycle, the heaviest ovarian weights (left and right) were recorded during diestrus, followed by metestrus, estrus then proestrus. The ovarian length, breadth and thickness showed the same pattern of variations with significant differences (P< 0.01). The largest average values recorded for ovarian length, breadth, thickness and weight were found in autumn followed by winter, spring and summer. Differences were significant in most of these criteria (P<0.01). The highest values recorded for the right ovarian weight noticed during autumn, in the diestrus phase of the cycle, (1.56±0.22g) while the lowest value was present in left ovary during summer in proestrus (0.79±0.52g). The right ovaries carried the heaviest C.L. and the larger numbers of follicles among all samples. The total number of all follicles (small, medium and large) is significantly higher (P< 0.01) in right than left ovaries (110.97±11.18 versus 106.38±13.09). The autumn has the largest values for the total numbers of follicles followed by winter, spring and summer. The maximum number of C. L. per doe reached 6, in single case, while in rare occasion a single ovary carried 4 C. L. In the majority of cases the ovary carried 1-2 C. L. The ratios between single C.L. weight and intact ovarian weight were 26.9, 24.6, 23.8 and 23.4 percentages during autumn, winter, spring and summer respectively.https://nvvj.journals.ekb.eg/article_177533_3d95e7fba335c5aee3b9244edf945f42.pdf