New Valley University, Faculty of Veterinary MedicineNew Valley Veterinary Journal2786-02721220210601Detection of Coliforms in table eggs1719434010.21608/nvvj.2021.194340ENSama'aMo'atazDepartment of food hygiene, Faculty of Veterinary Medicine, Assiut University.Nagah MSaadDepartment of food hygiene, Faculty of Veterinary Medicine, Assiut University,Tawfik AEl-BassionyDepartment of food hygiene, Faculty of Veterinary Medicine, Assiut University.Journal Article20210816The objective of this study was to determine the presence of coliforms, fecal coliform and Escherichia coli in both baladi and farm table eggs in both shells and contents. A total of 100 farms and baladi eggs samples (50 samples of each) were randomly collected from poultry farms, markets, supermarkets, and groceries in Assiut governorate. Eggs were microbiologically examined, and isolates were identified by biochemical and (PCR). The obtained results revealed that coliform and fecal coliform incidences were 62, 56, 54 and 32% for the farm hens' eggshell, egg content, baladi hens' eggshell and egg content, respectively. The biochemical identification of revealed 16 isolates of E. coli and these results were complementary with the molecular identification. E.coli incidence was 6, 8, 12 and 6% for the farm hens' eggshell, egg content, baladi hens' eggshell and egg content, respectively. The public health significance and hygienic control measures were discussed in this study.https://nvvj.journals.ekb.eg/article_194340_7f53fca31ee6b6d5c66edff1a38a97de.pdfNew Valley University, Faculty of Veterinary MedicineNew Valley Veterinary Journal2786-02721220210601Follicular Dynamic in Balady Goat in Upper Egypt Using Ultrasonography81420107710.21608/nvvj.2021.201077ENAhmed MOsmanDepartment of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Egypt.Hassan AHusseinDepartment of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Egypt.Mervat SHassanDepartment of Theriogenology, Faculty of Veterinary Medicine, New Valley University, New Valley, EgyptJournal Article20210911The objective is to study the Ultrasonographic appearances of the reproductive genitalia of cycling does (8 adult healthy, non-pregnant, cycling Balady does, examined daily for 6 weeks transrectal ultrasonographic examinations for two successive estrous cycles to investigate the length of cycle and as well the biometry of the ovaries, number and size of CL and follicles, size of uterus and cervix when possible). The obtained data showed that does have 3 or 4 Follicular waves. The last wave has the largest duration and the maximum follicle size (ovulatory). The characteristic features of follicular waves in does having three follicular waves denoted that the growth rates for the largest follicles of the ovulatory waves were significantly higher than first (p < 0.001) and second waves (p < 0.01). While, the characteristic features of follicular waves in doe having four follicular waves denoted that the growth rate of the largest follicles within each wave increased from the first to the fourth wave (0.49 to 0.85 mm/day respectively). The maximum diameter of the ovulatory follicles (7.83±0.51) were significantly larger than the maximum diameter of the largest follicles of the other waves (6.13±0.30 and 5.53±0.87 for third and first wave; P< 0.05), and with the second wave (5.33±0.40, p < 0.01). It is concluded that ultrasonographic examinations can be satisfactory performed in our small size local breed of Balady goats. Moreover, 3-4 follicular waves could be estimated per single cycle. However, the number of growing follicles per each wave decreased dramatically from the beginning of the wave to its end either with the anovulatory or ovulatory follicles.https://nvvj.journals.ekb.eg/article_201077_0d38d06978e12bbef291d302ddbf34a6.pdfNew Valley University, Faculty of Veterinary MedicineNew Valley Veterinary Journal2786-02721220210601Antibiotic resistance in bacteria isolated from the skin of trout in León Province, Spain152020336110.21608/nvvj.2021.203361ENJuan-MiguelFregeneda-GrandesDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, León, EspañaMohamed R.El SayedDepartment of Fish diseases and Management, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, EgyptHaitham H.SayedDepartment of Microbiology, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt.Journal Article20211002Antibiotic resistance among fish pathogens has become a major concern. Integrons play an important role in dissemination of antibiotics resistance genes among the different species of bacteria. This study was designed to determine the antibiotic susceptibility of 20 bacterial isolates were isolated from the skin of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss) collected from the rivers and fish farms in León Province, Spain and to detect class 1 and 2 integrons in these isolates by PCR to explore the role of these integrons in the antibiotic resistance. The investigated isolates included <em>Pseudomonas fluorescens (P. fluorescens)</em> (n=5), <em>Aeromonas sobria (A. sobria)</em> (n=3), <em>Pantoea agglomerans</em> (<em>P. agglomerans</em>) (n=3), <em>Acinetobacter johnsonii (A. johnsonii)</em> (n=2), <em>Serratia fonticola (S. fonticola)</em> (n=1), <em>Staphylococcus equorum (S. equorum)</em> (n=3), <em>Yersinia kristensenii (Y. kristensenii)</em> (n=2) and <em>Xanthomonas retroflexus (X. retroflexus)</em> (n=1). Antimicrobial susceptibility testing for the isolates revealed that they were sensitive to enrofloxacin (100%) and gentamicin (95%) while resistant to ampicillin (90%) and tetracycline (80%). PCR revealed that all the isolates didn’t harbor class 1 or 2 integrons. Therefore, the indiscriminate use of antibiotics must be restricted and more attention should be paid to biosecurity measures in aquaculture. Also, further studies are needed to assess role of integrons and other genetic elements in antimicrobial resistance in aquaculture.https://nvvj.journals.ekb.eg/article_203361_b59dddd5aa10b33a742b59fe07629854.pdfNew Valley University, Faculty of Veterinary MedicineNew Valley Veterinary Journal2786-02721220210601Prevalence of some deteriorating microorganisms in raw milk and some locally made cheese212720583810.21608/nvvj.2021.205838ENSally SGalabyDepartment of Food hygiene, Faculty of veterinary medicine, Assiut university, EgyptNagah Mohammed SaadMaharikDepartment of Food Hygiene , Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.Marwa IbrahimKhalifaDepartment of Food hygiene, Faculty of veterinary medicine, Aswan university, Aswan, Egypt.Journal Article20211016Food deterioration has become a very important issue as excessive amounts of food are lost due to microbial spoilage. The work aimed to assess the microbiological quality of raw milk and soft locally made cheeses in Assiut city, Egypt. A total of one hundred and fifty random samples of raw bovine milk (90 samples) and locally made cheese including Kareish and Domiati cheese (30 samples each) were collected randomly from different localities in Assiut city, Egypt. These samples were examined for microbiological evaluation. The investigation revealed that the average <em>APC</em> were 1.9×10<sup>6</sup>, 2.6×10<sup>6</sup>, 6.2×10<sup>6</sup>, 7.5×10<sup>5</sup> and 1.8×10<sup>5</sup> cfu/ml or /g for dairy farm milk, dairy shops, street vendor raw milk, Kareish cheese and Domiati cheese, respectively and the average <em>Psychrotrophic</em> count was 8.3×10<sup>5</sup>, 1.0×10<sup>6</sup>, 1.5×10<sup>6</sup>, 2.5×10<sup>5</sup> and 9.0×10<sup>4</sup> cfu/ml or /g while the average <em>Proteolytic</em> count was 1.6×10<sup>5</sup>, 2.1×10<sup>5</sup>, 3.7×10<sup>5</sup>, 5.3×10<sup>4</sup> and 2.0×10<sup>4</sup> cfu/ml or /g. Additionally, the average <em>Lipolytic</em> count was 2.5×10<sup>5</sup>, 3.9×10<sup>5</sup>, 6.5×10<sup>5</sup>, 9.8×10<sup>4</sup> and 2.8×10<sup>4</sup> cfu/ml or /g for the above-mentioned samples respectively. On the other hand, the <em>Lactobacillus</em> counts were 5.5×10<sup>4</sup>, 8.8×10<sup>4</sup>, 1.4×10<sup>5</sup>, 1.9×10<sup>4</sup> and 5.2×10<sup>3</sup> cfu/ml or /g, respectively. The average count of total yeast and molds were 9.5×10<sup>2</sup>, 2.5×10<sup>3</sup>, 7.5×10<sup>3</sup>, 7.5×10<sup>2</sup> and 6.2×10<sup>2</sup> cfu/ml or /g, respectively. In conclusion, the presence of these organisms indicted that the examined samples were of low quality which reflect unhygienic measures during milking, processing, storage, and distribution chain.https://nvvj.journals.ekb.eg/article_205838_d9b17f8ee13a64c9b44ae8db874d4a2d.pdf